Lamp1 staining
Tīmeklis2024. gada 2. janv. · OCN+OVA-fluorescein conjugate (ratio 3:1, green) were incubated with BMDMs. After the indicated incubation times at 37°C, cells were subjected to immunofluorescence staining using rat anti-LAMP1 antibody and anti-rat IgG conjugated Alexa Fluor ® 555 for lysosomes (red). Nuclei were stained with Hoechst … Tīmeklis2024. gada 23. febr. · (A, B) Immunofluorescence staining for either mTOR (green) and LAMP1 (red) and quantification was performed by counting the fluorescence intensity ratio of mTOR/LAMP1, n = 6/group. Scale bars = 10 μm. (C–E) Western blot images and analysis of phosphorylated AMPK, total AMPK and GPX4, n = 5/group. Data are …
Lamp1 staining
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Tīmeklis2005. gada 15. nov. · We chose to fuse GFP to human LAMP1, and not to the murine protein, because of the availability of a species-specific rat monoclonal antibody, … Tīmeklis2024. gada 31. janv. · Bottom: LAMP1 staining of full field (left) and higher magnification region with delineated zones (right) of liver. Right: Number of KFERQ-Dendra (top) and LAMP1 (bottom) positive puncta in liver ...
TīmeklisLAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Tīmeklis2024. gada 6. janv. · LAMP1 possesses a large luminal ectodomain and transmembrane domain, followed by a short C-terminal cytoplasmic tail (Fukuda 1991; Eskelinen et …
TīmeklisLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. TīmeklisIHC image of LAMP1 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with …
Tīmeklis2024. gada 3. sept. · Our study demonstrates that a significant portion of LAMP1-labeled organelles do not contain detectable lysosomal hydrolases including …
TīmeklisLAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment … giantex costway fridgeTīmeklis2024. gada 30. janv. · Confocal microscopy of the cerebellum confirmed that LAMP1 immunostaining was present in the calbindin-positive soma of the Purkinje neurons … giantex bunk bed full over fullTīmeklis2024. gada 13. apr. · Representative photomicrographs of double immunostaining of Lamp1 and Hsp70.1B in astrocytes (Lamp1: red; Hsp70.1B: green; Hoechst: blue). … frown clipartTīmeklis2024. gada 18. jūl. · LAMP1 (lysosomal associated membrane protein 1) and LAMP2 are distributed among autophagic and endolysosomal organelles. Despite … giantex costway washing machineTīmeklis2024. gada 23. jūl. · LAMP1 Antibody (1D4B) is an IgG2a rat monoclonal LAMP1 antibody that detects m, r, and h LAMP-1 by WB, IP, IF and FCM. 244 citations ... Western blot analysis, and immunostaining. Anti-LAMP1 Antibody (1D4B) is recommended for use in the following applications: WB (Western blotting), IP … giantex couchTīmeklisLAMP1 is a classic lysosomal marker and will work fine. Cite 6 Recommendations Top contributors to discussions in this field Margarethe Bittins University of Bergen J. Ramirez-Franco... frown catTīmeklisab25630 at 1/500 dilution staining LAMP1 in human gastric cancer cells by immunocytochemistry/ immunofluorescence. Sections were methanol fixed, permeabilized in 0.5% Triton X-100 prior to blocking in 10% NGS/1% BSA for 1 hour at 25°C and then incubated with ab25630 for 2 hours at 25°C. Alexa fluor® 594 mouse … giantex gym mat